Protein Method Blocking Buffers
MilliporeSigma™ Calbiochem™ BLOT-QuickBlocker™ Reagent
Prevents nonspecific proteins from binding to blots.
MilliporeSigma™ HAT Assay Buffer, 5X
For use in the HAT Assay.
MilliporeSigma™ Chemicon™ Choline Acetyltransferase Blocking Peptide
For Blocking AB143, AB144P Antibodies
Azure Biosystems Fluorescent Blot Blocking Buffer
Blocking solution for fluorescent Western blot, 1X solution, 500 mL
Azure Biosystems Chemi Blot Blocking Buffer
Blocking solution for chemiluminescent Western blot, 1X Solution
Azure Biosystems Protein-Free Blot Blocking Buffer
Protein-free blocking solution for Western blot, 5X concentrate
TBST (Tris-Buffered Saline + Tween 20), 20X Solution, Molecular Biology Grade, Hoefer™
Dilute to 1X with high purity H20 for a solution consisting of 25mM Tris, pH 7.4, 3.0mM KCl, 140mM NaCl and 0.05% Tween™ 20
LICOR Intercept (TBS) Protein-Free Blocking Buffer (500 mL)
Item is non returnable
Intercept (TBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays where animal-based products are prohibited. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no proteins, for assays where animal-based products are prohibited, it can be used with the widest range of targets for Western blot detection, it is ideal for primary antibodies that are optimized for Tris-based buffer systems, it can be used for detection of phosphorylated targets, it has superior performance for alkaline phosphatase detection, and it is an excellent choice for biotin-streptavidin detection.
Active Motif MAXblock™ Blocking Medium
MAXblock Blocking Medium is a protein-based, non-mammalian blocking agent for use in immunofluorescence and immunostaining assays. Superior blocking is achieved utilizing a protein blend that demonstrates no cross-reactivity with secondary antibodies. It has been proven effective at blocking non-specific binding of antibodies in immunofluorescence, as well as in immunohistochemistry.
Abcam 10 x Blocking Buffer, 50ML
10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding. - Cited in over 30 publications - Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB) - 100% casein blocking as preservative
The product is subject to the following: Abcam Restricted Use Statement